Number of Erythrocytes in Male Mice (Mus musculus) Exposed to Cigarette Smoke After Treatment with Ethanol Extract of Red Pomegranate Peel (Punica ganatu)

Abstract

Plant extracts have the potential to serve as natural therapeutic agents that can counteract the negative effects of various toxins, including those found in cigarette smoke. One such plant with this potential is the red pomegranate (Punica granatum). This study aims to determine the effect of administering Red Pomegranate (Punica ganatum) peel extract on the number of erythrocytes and hemoglobin levels in white mice exposed to cigarette smoke. This type of research is experimental laboratory post test only group design. Mice were divided into 4 treatment groups, namely: P0, control group, test animals treated with distilled water; treatment groups P1, P2 and P3, which were treated with neem leaf ethanol extract at a dose of 10 each; 20; 40 mg/head/day and mice were exposed to cigarette smoke. The research results showed that the higher the extract given, the higher the HB and erythrocyte levels. From this research it can be concluded that administration of Red Pomegranate rind extract can increase the number of erythrocytes and hemoglobin levels but it is not significant (p>0.05). The contribution of this research provides important data on the interaction between toxins (in this case, cigarette smoke) and protective agents (red pomegranate peel ethanol extract). This information is valuable in the fields of toxicology and pharmacology for understanding cellular protection mechanisms and potential new treatments.